I believe in blackborad-chalk method followed by notes, provided by me (lecture notes and prints out). For better understanding I also take animation sessions in biology lectures. All the lectures will be followed by test series and mock exams. I would like to take classes especially for MSc candidates.
I am PhD in Biotechnology. Taken classes for 6 years with 90 % success rate. Best for CSIR-UGC NET Life Sciences Courses. I have inspired my students for love of science and currently doing PhD at great institutes. Thank you.
Post Doctoral Fellow at IISER-Pune .
Project: Maharashtra Gene Bank which includes ecological interactions studies between marine sponges and their associated microorganisms.
Screening of anti fouling agents derived from marine sponge associated bacteria.
In addition, we are working on proteomics and mathematical modeling of marine bacterial predation and evolution of cancer cells among the normal cell populations.
Biophysical studies on bacterial aging using elastic light scattering experiments.
Mathematical modeling on how protozoa and other organisms cheats under anaerobic condition specifically in ruminants.
Identifying biosensor for heavy metal toxicity under marine environments.
1.) Srinu Meesala, Ruby Singh and Milind G. Watve . Anti-Mycobacterium Metabolite from Marine Sponge Iricinia fusca. British Journal of Pharmaceutical Research 15(4): 1-6, 2017.
2.) Srinu Meesala1, Ruby Singh, Milind G Watve, Dattatraya G. Naik. A new antibacterial imidazole from the marine sponge Iricinia fusca. Indian Journal of Natural Products and Resources.Vol. 9(1), March 2018.
2. Doctorate of Philosophy (Ph.D.) (July 2010-Dec 2016)
Title of thesis: Structure-function studies and evaluating the medical applications potential of two newly identified plant lectins
Thesis Guide: Dr. C G Suresh, Sr. Principal Scientist, Structural Biology Group, Biochemical Sciences Division, CSIR-National Chemical Laboratory, Pune, India
Isolation and Purification of plant lectins from various sources using column chromatography.
Biochemical characterization of the lectins based on hemagglutination activity and other assay monitored by UV-Vis spectroscopy and PAGE.
Investigating biophysical characteristics of lectin protein monitored by UV-Vis spectroscopy and steady state fluorescence spectroscopy.
Denaturant, pH and temperature induced folding – unfolding studies of lectins using steady state and time resolved fluorescence spectroscopy, circular dichroism spectroscopy, FT-IR, DLS and differential scanning calorimetry.
Determining the anti cancer activity of lectins against various human cancer cell lines. (Including apoptosis and angiogenesis assays)
Determining the 3-D structure of the lectins using X ray crystallography (Preliminary experience).
Ruby Singh, Laxman Nawale, Dhiman Sarkar, C. G. Suresh. Two Chitotriose-Specific Lectins Show Anti-Angiogenesis, Induces Caspase-9-Mediated Apoptosis and Early Arrest of Pancreatic Tumor Cell Cycle. Plos one. 2016 Jan 21;11(1):e0146110. doi: 10.1371/journal.pone.0146110.
Ruby Singh, Sushama M. Gaikwad and C. G. Suresh. A Chito-specific, Adenine Binding Agglutinin from Benincasa hispida Shows High Structural and Functional Stability. International Journal of Biochemistry Research & Review. Vol 9(4):1-14.
Ruby Singh and C. G. Suresh. Purification and Characterization of a Small Chito-specific Lectin from Datura innoxia Seeds Possessing Anti-microbial Properties. International Journal of Biochemistry Research & Review. Vol 9(2):1-17.
Ruby Singh, C G Suresh. Two chitotriose lectins showing broad spectrum antifouling activity against pre formed pathogenic biofilms (under review).
Ruby Singh, Sushama M. Gaikwad and C. G. Suresh. Structural characterization of a novel cytokinin binding agglutinin from Datura innoxia seeds and its acid-induced molten globule state (under review).
2. M.Sc. DISSERTATION THESIS: (Jan 2010-June 2010) Title: Study of extremophiles for their antimicrobial and anti oxidant properties" at Institute of Genomics and Integrative Biology (IGIB), New Delhi. Project Investigator: Dr. Hemant K Gautam, E II Scientist, Institute of Genomics and Integrative Biology (IGIB), New Delhi.
Isolating, purifying and identifying organisms from halophilic and psychrophilic samples
Cell culture and maintenance of the isolates.
Preparing whole cell lysate, extracting secondary metabolites using various solvents.
Studied antimicrobial and anti oxidant properties of the extract against MDR strains.
Additionally, designed primers for keratinase enzyme and cloned.
Rajiv Pathak, Ruby Singh, Avnika Singh, Hemlata Gautam, Atlar Singh Dhaker, Raj
Kumar, Rajesh Arora, Hemant K. Gautam. Assesment of a antibacterial and free radical scavenging activity in psychrophilic Arthrobactersp. Pharmacologyonline 1:344-355 (2011).
Animal cell culture: Setting up cell culture for primary as well as cancerous cell lines, quantification of cells in culture, MTT assay, Apoptotic assays, and Angiogenesis assays.
Molecular Biology: DNA and RNA isolation and purification, Plasmid isolation, Transformation, Restriction digestion, Ligation, Agarose gel electrophoresis, PCR, real time PCR, DNA sequencing, site directed mutagenesis.
Protein and Enzyme Biochemistry and Biophysics: Western Blotting, Immunoprecipitation, Column chromatography such as ion-exchange, gel filtration, FPLC, UV-Vis spectroscopy, Fluorescence spectroscopy, Time resolved fluorescence spectroscopy, Circular Dichroism spectroscopy, Differential Scanning Calorimetry, FT-IR, MALDI-TOF/TOF, LCMS analysis and Proteomics.
Microbiology: Isolation, Identification, Culture and maintenance of various bacterial and fungal strains.
Bioinformatics: Blast, ClustalW, Modeller (Homology modelling)
X ray crystallography, protein crystallization and data collection.
TEACHING JOB EXPERIENCE:
Assistant Professor at Department of Biotechnology in Modern College of Arts, Science and Commerce, Ganeshkhind, affiliated to Savitribai Phule University, from 15 July 2015 to April 2016.
Conducted project for second year BSc students, entitled “Phytochemical analysis and anti-biofilm activity of Muntingia calabura extract” and manuscript was published.
Publication: Singh R*, Iyer S, Prasad S, Deshmukh N, Gupta U, Zanje A, Patil S, Joshi S. Phytochemical Analysis of Muntingia calabura Extracts Possessing Anti-Microbial and Anti-Fouling Activities. International Journal of Pharmacognosy and Phytochemical Research 2017; 9(6); 826-832.
Lecturer (Biotechnology and Aptitude) for CSIR/UGC NET Coaching classes for post graduate level students at A Thinking place coaching classes, Pune (Feb 2014 to Oct 2016).
AWARDS AND FELLOWSHIPS:
Joint CSIR-UGC Senior Research Fellowship Award (2012).
Joint CSIR-UGC Junior Research Fellowship Award (2010). A prestigious award and recipient of merit scholarship through National Eligibility Test, conducted by Council of Scientific Industrial Research, Govt. of India.
Oral Presentation on “An era of living antibiotic: Bacterial Predation” at DST-SERB School on Chemical Ecology, conducted at NCBS Banglore, July 3-14, 2017.
Presented a poster on “Purification and characterization of an agglutinin from Datura innoxia with antineoplastic activity” on National Science Day, Feb 25-28, 2015, conducted in CSIR-NCL, Pune.
Presented a poster on “Biophysical Characterization of a highly thermostable phytoagglutinin from Benincasa hispida” on National Science Day, Feb 25-28, 2014 , conducted in CSIR-NCL, Pune
Participated in 42nd National Seminar on Crystallography and International Workshop on Application of X-ray diffraction for drug discovery, Nov 21-13, 2013, New Delhi.
Institute/ Board/ University
Year of Passing
Cochin University of Science and Technology (CUSAT)
FULL NAME : Dr. RUBY SINGH
SEX : FEMALE
DATE OF BIRTH : MAY 30th 1988
MARITAL STATUS : MARRIED
TELEPHONE : +91 (concealed information)
NATIONALITY : INDIAN
LANGUAGES KNOWN : ENGLISH, HINDI AND MALAYALAM
I don't think ruby maam is just a teacher she is a superskilled person. Thanks maam for making me to realize the enjoyment in studies, u leaved our institute by leaving a significant remark in a student's life
Ruby is one of those teachers who teaches beyond textbooks which is needed in today's world where the field of biology and science is growing everyday.I have been Ruby's student and have enjoyed learning with her.She has both experience and talent for a good teaching.
Dr. Ruby has been a colleague of mine at the Institute and she is very passionate about her subject. Her understanding of the subject is thorough which she articulate well with her excellent communication skills.
Dr.Ruby Singh is an excellent and the most trusted teacher. She excel in academics and has thorough conceptual and practical knowledge. she has a great teaching experience and most importantly lays her focus on all the students she teaches.I undoubtedly recommend her as she blend in with the quality of superprof faculty.
Very well versed with the basics. Has clarity in her explanations and makes learning fun.
Ruby ma'am is student friendly and she is very sincere.She explains the subject wonderfully well and doubt clearing sessions are the best with her.She is the assurance for 100 percent success.
Because, she teaches everything by Hart and make harder topic even simple tone understand.
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